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The measurement of ferritin in the leukemic blasts with a “sandwich” type enzyme immunoassay method
Author(s) -
Tanaka Masao,
Kato Kanefusa
Publication year - 1983
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19830101)51:1<61::aid-cncr2820510115>3.0.co;2-8
Subject(s) - immunoassay , ferritin , radioimmunoassay , acute monocytic leukemia , leukemia , medicine , enzyme , acute leukemia , serum ferritin , microbiology and biotechnology , immunology , chemistry , biochemistry , biology , antibody
The concentrations of ferritin in serum and leukemic blasts were measured with the improved method of enzyme immunoassay for ferritin which uses β‐D‐galactosidase from Escherichia coli . Precise measurement of ferritin was possible for concentration as low as 0.2 ng/ml serum. A good correlation (correlation coefficient, 0.946) existed between the ferritin radioimmunoassay and enzyme immunoassay. The serum ferritin levels in healthy males were 97.2 ± 46.7 ng/ml, whereas those in healthy females were 53.5 ± 27.1 ng/ml. Serum ferritin level was markedly increased (698 ± 447 ng/ml) in sera of 36 patients with various types of acute leukemia, of which acute monocytic leukemia showed the highest serum ferritin concentrations (1156 ± 324 ng/ml). Leukemic blasts from 24 patients with acute leukemia contained markedly elevated concentrations of ferritin (64.3 y 38.7 ng/10 6 cells) as compared with the ferritin concentration in normal leukocytes (7.1 ± 2.8 ng/10 6 cells). The blasts cells from the patients with acute monocytic leukemia showed the highest ferritin levels (105 ± 26.6 ng/10 6 cells).