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The glucose distribution in 9l rat brain multicell tumor spheroids and its effect on cell necrosis
Author(s) -
Li Conan K. N.
Publication year - 1982
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19821115)50:10<2066::aid-cncr2820501017>3.0.co;2-x
Subject(s) - spheroid , necrosis , distribution (mathematics) , cell , diffusion , tumor cells , kinetics , brain cell , biophysics , medicine , biochemistry , in vitro , biology , cancer research , microbiology and biotechnology , mathematical analysis , physics , mathematics , quantum mechanics , thermodynamics
The glucose consumption rate of 9L rat brain tumor cells was determined as a function of concentration. Glucose uptake followed Michaelis‐Menten kinetics with a K m of 0.58 mM and a v max of 1.6 pg/cellmin. The glucose diffusion coefficient in spheroids of 9L tumor cells was determined to be 1.5 × 10 −6 cm 2 /s at 37°C. Using these parametric values, the glucose distribution in 9L multicell spheroids was calculated and related to the viable and necrotic zones.