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The endodermal origin of the endocrine cells of an adenocarcinoma of the colon of the rat
Author(s) -
Cox W. F.,
Pierce G. Barry
Publication year - 1982
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19821015)50:8<1530::aid-cncr2820500813>3.0.co;2-9
Subject(s) - enteroendocrine cell , neural crest , adenocarcinoma , pathology , endocrine system , biology , cellular differentiation , endoderm , transplantation , cell , medicine , embryo , cancer , microbiology and biotechnology , endocrinology , genetics , gene , hormone
Abstract A transplantable adenocarcinoma of the colon of the rat, which contained mucous, columnar, endocrine, and undifferentiated carcinoma cells, were cloned to see if each of the differentiated cell lineages had a common cell or origin. Four clonal lines were produced by the transplantation of single cells using micropipettes. Each tumor contained all four cell lineages but in markedly differing proportions. A confirmatory experiment was performed using a lung colony cloning assay. Seven tumors were obtained; each had mucous, columnar, endocrine, and undifferentiated cells. It is concluded that the endocrine cells of the colon are derived by differentiation from endoderm. They are not of neural crest origin.