Premium
Clonogenic assay and in vitro chemosensitivity testing of human urologic malignancies
Author(s) -
Sarosdy Michael F.,
Lamm Donald L.,
Radwin Howard M.,
Von Hoff Daniel D.
Publication year - 1982
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19821001)50:7<1332::aid-cncr2820500719>3.0.co;2-8
Subject(s) - clonogenic assay , chemosensitivity assay , medicine , cancer , in vitro , pathology , cancer cell , cancer research , oncology , biology , biochemistry
Over the past year, we have attempted to grow both primary and metastatic urologic malignancies using a recently developed human tumor cloning system. Formation of colonies in vitro occurred in 125 of 164 primary tumors (76%), including 34 of 47 uroepithelial cancer specimens, 45 of 50 renal cell cancer specimens, 24 of 33 prostatic cancer specimens, and 22 of 34 testicular cancer specimens. A large percentage of metastatic cancers have also been successfully cultured. Growth sufficient for chemosensitivity testing ranged from 43% of the uroepithelial cancers cultured to 64% of the renal cell cancer specimens cultured. When in vitro chemosensitivity testing was performed, the in vitro chemosensitivity results show a striking similarity to clinical response rates for the same agents used for these tumors. Overall the human tumor cloning system appears to be a reasonable model for the study of human urologic malignancies.