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Colon‐specific antigen‐p (CSAp). II: Further characterization in colorectal and pancreatic cancer
Author(s) -
Shochat Dan,
Pant Keshab D.,
Goldenberg David M.
Publication year - 1982
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19820901)50:5<927::aid-cncr2820500521>3.0.co;2-0
Subject(s) - medicine , colorectal cancer , antigen , pancreatic cancer , cancer , gastroenterology , immunology
The physicochemical and immunological characteristics of colon‐specific antigen‐p (CSAp) in plasma and in colorectal and pancreatic tumors were investigated. CSAp in the plasma of a rectal cancer patient and in a colonic carcinoma xenografted in hamsters (GW‐39 tumor) appeared to have similar chromatographic properties, being of a molecular size of 4 million or more. The activities of CSAp in both plasma and tumor were similarly destroyed by treatment with a thiol reagent. Finally, identical immunological reactions in radioimmunoassay and gel diffusion tests were obtained between the CSAp's in patient plasma and in GW‐39 tumor tissue. Also, CSAp in human pancreatic cancers xenografted in nude mice showed a precipitin line of complete identity with CSAp extracted from GW‐39 human colonic carcinoma transplants. Thus, the CSAp's found in colorectal cancer patient plasma, in colonic carcinoma, and in pancreatic cancer tissues appear to be immunologically identical.