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Sensitivity to 1‐(4‐amino‐2‐methyl‐5‐pyrimidinyl)methyl‐3‐(2‐chloroethyl)‐3‐nitrosourea hydrochloride (ACNU) of glioma cells in vivo and in vitro
Author(s) -
Yoshida Jun,
Shibuya Naoki,
Kobayashi Tatsuya,
Kageyama Naoki
Publication year - 1982
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19820801)50:3<410::aid-cncr2820500305>3.0.co;2-3
Subject(s) - nitrosourea , in vivo , medicine , cell culture , in vitro , glioma , cell growth , cell , cancer research , microbiology and biotechnology , pharmacology , biochemistry , biology , chemotherapy , genetics
A new water‐soluble nitrosourea (ACNU) was tested for its antitumor activity against four glioma cell lines. Four factors were studied to determine its antitumor activity: inhibition of cell growth, morphologic observation, analysis of DNA histogram with flow microfluorometry, and sensitivity testing with microtest plate. Growth inhibition in response to ACNU was seen in two cell lines (EA285, U251‐MG), whereas two cell lines (YE2‐2, T98) were resistant to ACNU. The results of the present sensitivity test concur with those of other examinations that this test is useful in selecting a drug and determining the effective dose.