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Immunological characterization of non‐Hodgkin's lymphoma: Enrichment of neoplastic cells from lymphoid tissues and blood
Author(s) -
BomVan Noorloos Adri A.,
Van Beek Agaath A. M.,
UbelsPostma José,
Zeijlemaker W. P.,
Van Heerde P.,
Von Dem Borne A. E. G. Kr.,
Cleton F. J.,
Melief C. J. M.
Publication year - 1982
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19820415)49:8<1605::aid-cncr2820490813>3.0.co;2-p
Subject(s) - lymphoma , population , medicine , pathology , spleen , lymph , cell , b cell , non hodgkin's lymphoma , neoplastic cell , immunology , monoclonal , monoclonal antibody , biology , antibody , environmental health , genetics
Cell suspensions prepared from lymph nodes, spleen or peripheral blood of patients with non‐Hodgkin's lymphoma (NHL) often contain a high percentage of residual nonmalignant cellular elements. By Erosette sedimentation, it was possible to enrich such suspensions from patients with various types of lymphoma for malignant cells. In patients with a B‐ or non‐B/non‐T‐cell lymphoma, the neoplastic cells were found in the non‐T fraction. The capacity to respond to stimulation by various stimuli was then confined to the T‐cell fraction, which contained the residual normal T‐cells. In patients with T‐cell lymphomas, in which the malignant cells had retained the capacity to form E‐rosettes, lymphoma cells were found in the T fraction. These cells usually did not respond to mitogenic stimuli. Using this separation method, small proportions of neoplastic cells could be identified in mixed cell populations. Thus, in the blood from nine out of 23 lymphoma patients without abnormalities in routine blood tests, a population of abnormal cells was detected after cell separation. This included a monoclonal B‐cell population in the blood of four patients, a questionably monoclonal B‐cell population in the blood of two patients and an increased non‐B/non‐T cell population in the blood of three patients.