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In vitro growth of lymphoma colonies from children with non‐hodgkin's lymphoma
Author(s) -
Smith Stephen D.,
Wood Gary W.,
Fried Paula,
Lowman James T.
Publication year - 1981
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19811215)48:12<2612::aid-cncr2820481213>3.0.co;2-r
Subject(s) - lymphoma , lymphoblastic lymphoma , cell culture , pathology , bone marrow , null cell , in vitro , myeloid , medicine , agar , cell , malignant lymphoma , biology , cancer research , immunology , t cell , biochemistry , immune system , bacteria , genetics
Growth in vitro of malignant lymphoid cells has been previously reported, but those studies involved direct establishment of cell lines from the patients' cells. In the present study, an in vitro system for both primary growth and passage of malignant lymphoblastic colony‐forming cells (ML‐CFC) was established, and for the first time, colony‐forming cells from non‐Hodgkin's lymphoma patients were cultured, and cell lines were established from individual colonies. Malignant lymphoid colonies grew from 9 of 16 (56%) bone marrow aspirates, which were histologically involved with lymphoma and from 5 of 29 (17%) morphologically normal bone marrow samples. ML‐CFCs grew concomittantly with myeloid and monocytic CFCs and were distinguishable by their morphologic characteristics on agar, cytochemical staining characteristics, and cell surface markers. Successful passage of ML‐CFCs was accomplished in five patients, and ten distinct cell lines (six null cell, three B cell, and one T cell) were established. This agar assay system provides an opportunity to study malignant lymphoma cell growth both as a primary colony and as a cell line established from a primary colony.