Premium
Dissociation in the response of the adenylate cyclase system to thyrotropin and prostaglandin E 2 in human thyroid carcinoma tissue
Author(s) -
Arcangeli Paolo,
Toccafondi Roberto,
Rotella Carlo M.,
Aterini Stefano,
Tanini Annalisa,
Borelli Domenico,
Loddi Loddo
Publication year - 1981
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19810801)48:3<757::aid-cncr2820480316>3.0.co;2-v
Subject(s) - endocrinology , medicine , cyclase , adenylate kinase , thyroid , adenoma , prostaglandin e2 , prostaglandin , involution (esoterism) , follicular phase , receptor , biology , consciousness , neuroscience
Because the existence of a damaged thyrotropin (TSH) receptor in thyroid tumors may be relevant in the perspective of a correct postsurgical therapy, the effect of TSH on cAMP intracellular accumulation in thyroid carcinoma (N = 16), follicular adenoma (N = 27) and normal tissue (N = 30) slices was studied and compared with that of nonspecific stimulus of thyroid adenylate cyclase‐cAMP system, such as prostaglandin E 2 (PGE 2 ). While in all follicular adenomas a normal behavior of basal and post‐TSH and ‐PGE 2 stimulated cAMP accumulation was observed, basal cAMP levels were generally higher than in controls in 14 differentiated carcinomas, responses to TSH were reduced or absent, and response to PGE 2 was close to normal. On the contrary, in two anaplastic carcinomas, both TSH and PGE 2 produced a negligible modification of cAMP levels. Thus, in undifferentiated carcinomas, the adenylate cyclase‐cAMP system seems to be altered; in differentiated carcinomas, the catalytic part of the system appears unaffected as it is PGE 2 ‐responsive. Therefore, some hypotheses are ruled out as an explanation for decreased sensitivity to TSH of differentiated carcinomatous cells.