Premium
The DNA content of human plasma cells
Author(s) -
Brox Larry,
Mowles Dolores,
Pollock Betty,
Belch Andrew
Publication year - 1981
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19810515)47:10<2433::aid-cncr2820471020>3.0.co;2-d
Subject(s) - propidium iodide , feulgen stain , dna , pathology , plasma cell , flow cytometry , multiple myeloma , fluorescent staining , staining , medicine , ploidy , microbiology and biotechnology , biology , apoptosis , bone marrow , biochemistry , gene , programmed cell death
The DNA content of human plasma cells from myeloma patients relative to that of leukocytes was determined by flow and microscopic cytofluorometry after propidium iodide and fluorescent Feulgen staining, respectively. Mononucleated myeloma plasma cells from all of the 17 patients studied contained more DNA (17 to 58%) than the leukocytes from the same patient. The binucleated and trinucleated plasma cells, which were more prevalent in advanced cases, contained up to two and three times, respectively, the amount of DNA determined in the mononucleated plasma cells. These observations suggest that the ploidy abnormalities of myeloma plasma cells are even more extensive than the numerous karyotypic studies have indicated.