Premium
Transplantable pancreatic acinar carcinoma
Author(s) -
Warren John R.,
Reddy Janardan K.
Publication year - 1981
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19810315)47:6+<1535::aid-cncr2820471416>3.0.co;2-1
Subject(s) - endoplasmic reticulum , acinar cell , golgi apparatus , secretagogue , medicine , endocrinology , pancreas , intracellular , acinus , biology , chemistry , microbiology and biotechnology , secretion
Fragments of the nafenopin‐induced pancreatic acinar cell carcinoma of rat have been examined in vitro for patterns of intracellular protein transport and carbamylcholine‐induced protein discharge. Continuous incubation of the fragments with [ 3 H]‐leucine for 60 minutes resulted in labeling of rough endoplasmic reticulum, Golgi cisternae, and mature zymogen granules, revealed by electron microscope autoradiography. This result indicates transport of newly synthesized protein from the rough endoplasmic reticulum to mature zymogen granules in approximately 60 minutes. The secretagogue carbamylcholine induced the discharge of radioactive protein by carcinoma fragments pulse‐chase labeled with [ 3 H]‐leucine. A maximal effective carbamylcholine concentration of 10 −5 M was determined. The acinar carcinoma resembles normal exocrine pancreas in the observed rate of intracellular protein transport and effective secretagogue concentration. However, the acinar carcinoma fragments demonstrated an apparent low rate of carbamylcholine‐induced radioactive protein discharge as compared with normal pancreatic lobules or acinar cells. It is suggested that the apparent low rate of radioactive protein discharge reflects functional immaturity of the acinar carcinoma. Possible relationships of functional differentiation to the heterogeneous cytodifferentiation of the pancreatic acinar carcinoma are discussed.