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Morphologic and biochemical characteristics of isolated and cultured pancreatic ducts
Author(s) -
Githens Sherwood,
Holmquist Doris R. G.,
Whelan Joelle F.,
Ruby John R.
Publication year - 1981
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19810315)47:6+<1505::aid-cncr2820471411>3.0.co;2-j
Subject(s) - hamster , collagenase , alkaline phosphatase , in vitro , chymotrypsin , pancreatic duct , in vivo , trypsin , acinus , biology , biochemistry , microbiology and biotechnology , enzyme , pancreas
Rat and hamster pancreatic ducts were isolated by digestion with collagenase plus chymotrypsin and were cultured for eight weeks in an agarose matrix. Freshly isolated and cultured ducts were characterized morphologically and biochemically. The in vivo morphology of the ducts was maintained in vitro , although certain differences were noted. Both interlobular and intralobular ducts could be identified. γ‐Glutamyltranspeptidase and Mg‐ATPase were stable enzymatic activities of the ducts of both species; alkaline phosphatase persisted only in the hamster ducts. Carbonic anhydrase and (Na + K)ATPase were minor activities of the rat ducts. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of the rat ducts suggested that actin was the major duct peptide and that the major zymogens were greatly diminished. These results demonstrate that pancreatic ducts can be maintained in vitro and can be used for biochemical studies of this minor pancreatic tissue type.