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A review of in vitro and in vivo culture techniques for the study of pancreatic carcinogenesis
Author(s) -
Jones Raymond T.,
Hudson Eric A.,
Resau James H.
Publication year - 1981
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19810315)47:6+<1490::aid-cncr2820471409>3.0.co;2-5
Subject(s) - medicine , in vivo , carcinogenesis , in vitro , cancer research , cancer , biology , microbiology and biotechnology , genetics
Techniques have been developed for long‐term organ explant culture of bovine, hamster, and human pancreatic ducts in enriched medium. The explants were then exposed to chemical carcinogens and studied by morphologic and biochemical techniques. Untreated explants have also been successfully xenotransplanted into athymic “nude” mice. Techniques have also been developed to isolate human and bovine pancreatic ductal cells for cell culture. These as well as techniques developed by other authors are discussed.