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Activity and isoenzymes of acid phosphatase in human B‐cell lymphomas of low‐grade malignancy: A novel aid in the classification of malignant lymphoma
Author(s) -
Schmidt Dietmar,
Radzun Heinz J.,
Schwarze ErnstW.,
Stein Harald,
Parwaresch Mohammad R.
Publication year - 1980
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19801215)46:12<2676::aid-cncr2820461223>3.0.co;2-l
Subject(s) - malignant lymphoma , medicine , malignancy , isozyme , lymphoma , cancer research , b cell , acid phosphatase , oncology , pathology , immunology , enzyme , biochemistry , biology , antibody
Activity and isoelectric focusing (IEF) pattern of lysosomal acid phosphatase (E.C.3.1.3.2.) were investigated in 55 cases of low‐grade malignant B‐cell lymphoma, classified as chronic B‐lymphocytic leukemia (B‐CLL), centroblastic/centrocytic follicular lymphoma (CB/CC), lymphoplasmacytic/lymphoplasmacytoid lymphoma (Immunocytoma, IC), and plasmacytoma (PC), applying the criteria of the Kiel classification. The results show (1) that the four lymphoma types present a characteristic range of enzyme activity in an increasing order: B‐CLL, CB/CC, IC, and PC. B lymphocytes, germinal center cells, and plasmacytes are the main constituents of these lymphomas. This sequence might reflect one possible mode of B‐cell transformation into plasmacytes traversing an amplification stage in germinal centers under normal conditions. (2) All cases showed the basic IEF pattern of normal B lymphocytes with 12 bands localized in three regions between pH 6.1 and 3.9. This finding supports the B‐cell origin and the close phenotypical relationship among the investigated lymphomas. (3) The IEF patterns of B‐CLL and CB/CC did not differ from that of normal B lymphocytes, whereas two additional isoenzymes were encountered in cases of IC and seven in PC; this suggests that the higher enzyme activity of IC and PC is at least partly due to the appearance of “new” isoenzymes. The results support the validity of the underlying classification and indicate the individuality, B‐cell origin, and close relationship among the four lymphoma entities investigated.