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Progesterone receptors: Saturation analysis by a solid phase hydroxylapatite adsorption technique
Author(s) -
Hoffman P. G.,
Jones L. A.,
Kuhn R. W.,
Siiteri P. K.
Publication year - 1980
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19801215)46:12+<2801::aid-cncr2820461412>3.0.co;2-w
Subject(s) - hydroxylapatite , receptor , saturation (graph theory) , progesterone receptor , ligand (biochemistry) , cytoplasm , adsorption , medicine , chromatography , chemistry , biochemistry , breast cancer , cancer , estrogen receptor , mathematics , combinatorics , enzyme
An assay for cytoplasmic progesterone receptor is described in which receptor is bound to hydroxylapatite and is washed before saturation analysis. Using 3 H‐progesterone as ligand, the assay yields lower, more reproducible nonspecific binding than is obtained with dextran‐coated charcoal techniques and may provide a clinically useful method of measuring progesterone receptor in breast carcinoma. Nuclear progesterone receptors can also be determined by the same technique.