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Human yolk sac tumor serially transplanted in nude mice: Its morphologic and functional properties
Author(s) -
Hata JunIchi,
Ueyama Yoshito,
Tamaoki Norikazu,
Akatsuka Akira,
Yoshimura Shinichi,
Shimuzu Koichi,
Morikawa Yukihiko,
Furukawa Toshiharu
Publication year - 1980
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19801201)46:11<2446::aid-cncr2820461125>3.0.co;2-z
Subject(s) - pathology , yolk sac , immunoelectron microscopy , golgi apparatus , transplantation , nude mouse , endoplasmic reticulum , hemopexin , transferrin , biology , albumin , immunohistochemistry , medicine , microbiology and biotechnology , cell culture , embryo , endocrinology , biochemistry , heme , genetics , enzyme
Three human yolk sac tumors (TE, OE, and TT‐1) were serially transplanted into nude mice. The histology of the transplanted tumors is moderately different from that of the original tumor: the transplants had mainly a tubular structure with an inconspicuous endodermal sinus structure due to a decreased mesenchymal element surrounding the vascular core. Ultrastructural features of the transplanted tumor cells, however, were identical to those of the original tumor. Immunochemical survey of the blood plasma of nude mice bearing these tumors demonstrated the production of adult type human plasma proteins including prealbumin, albumin (Alb), α,‐antitrypsin (αAT), transferrin (Tf), hemopexin, and α1‐fetoprotein (AFP). The types of adult plasma proteins that were produced varied, depending on the tumor line but were the same between serial passages within the same tumor line. The intracellular localization of AFP, αAT, Tf, and Alb was also investigated by immunoelectron microscopy. Plasma proteins of the adult type and AFP were localized mainly within the membrane systems of the endoplasmic reticulum and Golgi complex and occasionally on the microvilli. These results suggest that transplanted human yolk sac tumors have almost all of the protein‐producing function possessed by their normal counterparts.