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Discriminative value of isozymes of amino acid naphthylamidase in the diagnosis of myeloid leukemias
Author(s) -
Roos Göran,
Sinna Gamal Abu,
Björkstén Bengt,
Lenner Per,
Lundgren Erik
Publication year - 1980
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19800715)46:2<325::aid-cncr2820460219>3.0.co;2-j
Subject(s) - isozyme , medicine , myelofibrosis , myeloid , myeloid leukemia , polycythemia vera , amino acid , electrophoresis , clone (java method) , haematopoiesis , enzyme , immunology , biochemistry , biology , genetics , gene , bone marrow , stem cell
Isozymes of amino acid naphthylamidase (called B and C) with deviating electrophoretic mobilities were found in peripheral blood leukocytes of 25 out of 25 untreated acute and chronic myeloid leukemias (AML and CML) and in 2 out of 2 cases of idiopathic myelofibrosis, while a normal pattern was found in 3 control groups and 5 cases of polycythemia vera. In the AML group, a correlation between electrophoretic mobility and the number of blast cells was found, and on remission, the B isozyme mobility was nearly normalized. Thus, deviating electrophoretic mobility of the B isozyme seemed to be valuable for diagnosis of myeloid leukemias, and in the AML group, the change in mobility might indicate the size of the leukemic clone.