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Chromosome analysis of primary large bowel tumors. A new method for improving the yield of analyzable metaphases
Author(s) -
Martin P.,
Levin B.,
Golomb H. M.,
Riddell R. H.
Publication year - 1979
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197911)44:5<1656::aid-cncr2820440519>3.0.co;2-z
Subject(s) - karyotype , chromosome , chromosome analysis , yield (engineering) , medicine , resection , pathology , biology , genetics , surgery , physics , gene , thermodynamics
Colon tumors were processed immediately after surgical resection. Using L‐Arterenol and sodium citrate in combination with standard chromosome culture techniques, 12 of 17 consecutive tumors (75%) had countable figures ranging from 5 cells to 59 cells. Seven of the 12 cases had over 25 countable cells. Chromosome counts did not vary more than ±4 for any one case. Seven tumors were karyotyped, 6 with banding techniques. One of these specimens had mosaic patterns. The chromosome counts varied: 2 were hypodiploid; 1 was pseudodiploid and 4 were hyperdyploid. There were some similarities in the karyotypes although no identical karyotypes were seen in this series.