Premium
Immunodiagnosis of mesothelioma. Use of antimesothelial cell serum in an indirect immunofluorescence assay
Author(s) -
Singh Gurmukh,
Whiteside Theresa L.,
Dekker Andrew
Publication year - 1979
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197906)43:6<2288::aid-cncr2820430619>3.0.co;2-n
Subject(s) - antiserum , mesothelial cell , serous fluid , mesothelioma , immunofluorescence , pathology , medicine , mesothelium , in vitro , indirect immunofluorescence , cell culture , cell , microbiology and biotechnology , biology , immunology , antibody , biochemistry , genetics
Cells isolated from human serous effusions were cultured in vitro. Monolayers of large multipolar cells were established. Antisera to the cultured cells were prepared in rabbits and rats. The antisera were absorbed with human red cells, liver powder and MOLT‐4F cell line lymphocytes. Specificity of the absorbed antisera for human mesothelial cells was demonstrated in an indirect immunofluorescence assay. The antisera were used to confirm the diagnosis of mesothelioma in two cases. In both the patients, the morphologically identifiable malignant cell populations in the effusions stained positively with the anti‐mesothelial cell serum thus establishing their mesothelial origin. Normal nonmesothelial tissue and known nonmesothelial tumors failed to react with the antisera thus confirming the specificity of the antisera.