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On the presence of reverse transcriptase in myelo‐ and lymphoproliferative disorders
Author(s) -
Van Muijen G. N. P.,
Velde J. Te,
Den Ottolander G. J.,
Brand A.,
KoopmanBroekhuyzen N.,
Schaberg A.,
Warnaar S. O.
Publication year - 1979
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197905)43:5<1682::aid-cncr2820430519>3.0.co;2-0
Subject(s) - buffy coat , medicine , reverse transcriptase , malignancy , leukemia , lymphoproliferative disorders , hematological malignancy , virology , hematological disorders , pathology , immunology , lymphoma , biology , rna , biochemistry , gene
Buffy coats from 31 patients with a diagnosis of leukemia and 16 normal donors were tested for the presence of a viral‐like reverse transcriptase. Eighty‐five percent of fresh leukemic buffy coats were positive. Also tested were spleens from 16 patients with hematological disorders and 5 spleens from patients without history of hematological malignancy. The 5 normal spleens were negative. Also negative were 4 spleens from patients with Hairy cell leukemia. From the remaining 12 spleens 7 were positive. Reverse transcriptase measurements can be used to distinguish leukemic from normal buffy coats.