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Cellular content of carcinoembryonic antigen in urothelial carcinoma
Author(s) -
Wahren Britta
Publication year - 1978
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197809)42:3+<1533::aid-cncr2820420827>3.0.co;2-t
Subject(s) - carcinoembryonic antigen , antiserum , pathology , staining , medicine , cell , carcinoma , antigen , biology , cancer , immunology , genetics
CEA containing cells can be demonstrated in 25–60% of urothelial carcinomas. No staining was seen with benign urothelial cells. Using microfluorometry of single cells, mean fluorescence intensity with anti‐CEA antisera was 3–6 times that of preparations stained in parallel with nonimmune sera. A comparison of the IF results with RIA implies a possibility to detect <1 pg CEA/ cell in unfixed specimens. The cells which stained with anti‐CEA antisera did not have any apparent morphologic properties differentiating them from neighboring nonstained cells but it was mainly in the cell populations from well‐ and moderately well‐differentiated tumors that CEA containing cells were seen. Therefore quantitative measurements of CEA amount/cell may be a parameter, in addition to morphologic differentiation, to study new properties of tumor cell populations.