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Ultrastructural investigation of NI 3 S 2 ‐induced rhabdomyosarcoma in wistar rat. Comparative study with emphasis on myofibrillar differentiation and ciliar formation
Author(s) -
Hildebrand Hartmut F.,
Biserte Gérard
Publication year - 1978
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197808)42:2<528::aid-cncr2820420222>3.0.co;2-d
Subject(s) - myofilament , myofibril , ultrastructure , sarcomere , anatomy , electron microscope , mitosis , biology , centriole , myocyte , rhabdomyosarcoma , microtubule , microbiology and biotechnology , pathology , medicine , physics , biochemistry , sarcoma , optics
Nickel‐sulfid‐induced rhabdomyosarcomas were studied by both light and electron microscopy. The successive stages of differentiating tumor cells were described, and two differentiation types of rhabdomyoblasts could be observed 1) with the characteristic pattern of fetal differentiation— i.e. , myofilament apparition before Z‐line formation—and 2) with synthesis of these elements in the reverse order. An organized T‐system is not evident. The sarcoplasmic reticulum is irregular and its cisternae often contain a granular substance or microcrystals. The only well‐developed element of tumoral myofibrils is the Z‐line the other zones of sarcomeres are seldom clearly defined. Several unusual granular structures were observed. No virus particles were found. The formation of cilia appears only in interphase rhabdomyoblasts and has to be considered as aberrant and temporary formations from centrioles. They generally possess a “9 + 0” microtubular pattern, but often could be observed as rudimentary forms with a “7 + 2” microtubular arrangement. This case is another example demonstrating the relationship of cilia formation with cell division, especially after suppression of mitotic control. The histology and the electron microscopy results are discussed in relation to the differentiation pattern and the ultrastructural features of embryonic, regenerating and pathological muscle and muscle differentiating in vitro .