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Two‐dimensional polyacrylamide gel analysis of nuclear proteins from human tumor cell lines
Author(s) -
Yeoman L. C.,
Taylor C. W.,
Woolf L. M.,
Busch H.
Publication year - 1978
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197808)42:2<474::aid-cncr2820420215>3.0.co;2-f
Subject(s) - hela , polyacrylamide gel electrophoresis , microbiology and biotechnology , nuclear protein , chromatin , cell , gel electrophoresis , biology , staining , cell culture , nuclear matrix , cell nucleus , biochemistry , dna , nucleus , genetics , gene , transcription factor , enzyme
Two‐dimensional polyacrylamide gel electrophoretic analysis was carried out on HeLa, KB, ALL and GW‐39 cell “Chromatin Fraction II” proteins. Of the many proteins found, most were visualized in earlier studies on rodent tumors and normal tissues. Of these the greater density of protein CP and the presence of protein CG' differentiates tumors from the nontumor tissues. In samples of normal and mitogen stimulated human lymphocytes, protein CG' was absent and protein CP was present in small amounts. Two‐dimensional patterns of 0.4N H 2 SO 4 soluble nuclear proteins showed elevated amounts of proteins C16–18 in the GW‐39 cell patterns. Proteins Hul, G1, G2, G3 and G5 were detected only in human cell nuclei. The increased density of staining for protein CP and the presence of CG' are potential biological markers for neoplastic cells.