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A rapid in vitro method for measuring cell proliferation in human breast cancer
Author(s) -
Sklarew Robert J.,
Hoffman Joseph,
Post Joseph
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197711)40:5<2299::aid-cncr2820400542>3.0.co;2-#
Subject(s) - breast cancer , in vitro , collagenase , in vivo , human breast , medicine , incubation , cell growth , pathology , cancer cell , cancer , cell , cell culture , cancer research , microbiology and biotechnology , chemistry , biology , biochemistry , enzyme , genetics
A method has been developed for studying in vitro the cell proliferation kinetics of human breast cancer. Surgical specimens from primary tumors were studied in 56 patients. Viable cell suspensions for assay were obtained by the dissociation of tumor tissue with collagenase. Mean Labeling indices of 2.43 ± S. D. 2.05 and 4.48 ± S.D. 3.73, respectively, were found after incubation with 3 HTdR for 2 hours and 24 hours. Mean S‐times of 21.9 ± S.D. 4.3 hours were estimated by 3 H and 14 C‐TdR double‐labeling. The kinetic data have been validated by parallel labeling studies in vivo and in vitro in four patients. The processing of autoradiographs using gold latensification provided slides for kinetic analysis within 3 days. The assay offers a method that is useful in the planning and monitoring of drug therapy.