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Restoration of in vitro growth control to malignant cells
Author(s) -
Lipkin George,
Knecht Margarete E.,
Rosenberg Martin
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197711)40:5+<2699::aid-cncr2820400943>3.0.co;2-k
Subject(s) - hamster , in vitro , melanocyte , contact inhibition , microgram , melanoma , cell culture , hela , cell , epidermal growth factor , cell growth , cancer research , malignant cells , biology , microbiology and biotechnology , pathology , medicine , cancer , biochemistry , genetics
A glycoprotein (molecular weight, ca. 160,000) from culture medium of contact‐inhibited hamster melanocytes restores contact inhibition of growth to malignant melanocytes of man, mouse, and hamsters, and also effectively inhibits growth in vitro of a broad spectrum of malignant and normal cell types of ectodermal, mesodermal and endodermal origins, including human colon carcinomas. The melanocyte contact inhibitory factor (MCIF) produces G 1 growth arrest in malignant melanocytes; inhibition of all cell types is reversible, dose‐related, and nontoxic at concentrations below 200 μg/ml, but selectively lethal to malignant cells at higher concentrations. An electrophoretically identical protein is present in culture media of contact‐inhibited melanocytes, fibroblasts, and epidermal cells, but absent from those of colon carcinomas, HeLa cells and malignant melanomas. Nevertheless, an MCIF‐like band is present in whole cell homogenates of human colon carcinomas and hamster melanomas. MCIF may permit normal surface interactions required for feedback inhibition of growth.