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Changes in chromosomal proteins in colon cancer. The complexity and DNA‐binding properties of tumor‐associated proteins and evidence for their association with the malignant state in human colonic epithelium
Author(s) -
Boffa Lidia C.,
Allfrey Vincent G.
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197711)40:5+<2584::aid-cncr2820400930>3.0.co;2-c
Subject(s) - dna , chromatin , carcinogenesis , homologous chromosome , biology , dna binding protein , gene , non histone protein , cancer , microbiology and biotechnology , genome , cancer research , genetics , transcription factor
Abstract The properties of two classes of nonhistone nuclear proteins (NHNP) whose appearance has been correlated with the process of carcinogenesis in the colonic epithelium of rats given 1,2‐dimethylhydrazine (DMH) have been investigated. NHNP isolated from adenocarcinomas of the colon were tested for their binding to homologous DNA. The tumor‐specific protein class TNP 1 (molecular weight ca. 44,000) shows high affinity for DNA while the second class of tumor‐specific proteins (TNP 2 ; molecular weight ca. 62,000) does not bind to DNA. When tumor chromatin is subjected to a limited digestion with DNAse I under conditions that are known to preferentially digest active genes, 19 the TNP 1 proteins are selectively released, suggesting that this protein fraction is associated with the actively transcribing portions of the genome. The complexity of both tumor‐specific protein classes has also been analyzed by two‐dimensional gel electrophoresis. This method reveals a high degree of complexity both in TNP 1 and TNP 2 . Protein classes similar to TNP 1 and TNP 2 that are also found in human colonic adenocarcinomas are not detectable in polyps from familial polyposis‐affected patients at times when no sign of malignancy has yet appeared.