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Direct immunochemical detection of prostaglandin‐e and cyclic nucleotides in human malignant tumors
Author(s) -
Husby Gunnar,
Strickland Robert G.,
Rigler Gerald L.,
Peake Glenn T.,
Williams Ralph C.
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197710)40:4<1629::aid-cncr2820400436>3.0.co;2-s
Subject(s) - medicine , nucleotide , pathology , cancer research , biochemistry , biology , gene
Immunofluorescent localization of prostaglandin‐E (PGE), cyclic AMP (cAMP), and cyclic GMP (cGMP) was studied in tumor tissues from 40 patients with a variety of solid tumors. Representative normal tissues served as controls. Rabbit antisera specific for PGE or the cyclic nucleotides were used, and the reactions observed were correlated with the degree and type of lymphocytic reaction at the tumor margins. Strong PGE immunofluorescence was detected in tumor cells in 27 of 42 malignancies; by contrast nine of 13 normal tissues showed weak PGE reactions, cAMP was detected in 30 of the 42 malignancies; cGMP was noted in only seven of the 42 malignant tissues and in none of the normal tissues studied. The most common malignant tumor profile (17/42) was that of positive PGE and cAMP and negative cGMP staining. Tumors showing strong staining with anti‐PGE or cAMP demonstrated a distinct trend towards heavier lymphocytic infiltration with a predominance of T cells at their margins, although this association did not reach statistical significance in the present material.