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Radioimmunoassay for tumor antigen of human cervical squamous cell carcinoma
Author(s) -
Kato Hiroshi,
Torigoe Tadashi
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197710)40:4<1621::aid-cncr2820400435>3.0.co;2-i
Subject(s) - radioimmunoassay , antiserum , antigen , ouchterlony double immunodiffusion , medicine , heterologous , immunodiffusion , pathology , cervical cancer , carcinoma , cancer , immunology , biology , biochemistry , gene
A heterologous antiserum for human cervical squamous cell carcinoma was prepared and specificity determined by Ouchterlony immunodiffusion and immunofluoresence studies. With this antiserum, a tumor antigen was purified from human cervical squamous cell carcinoma tissue. The specificities of the antigen and the antiserum were then re‐examined by a radioimmunoassay method using 125 I‐labeled purified antigen. Although normal cervical tissue extract showed a moderate cross‐reactivity in the radioimmunoassay, the circulating antigen activity could not be detected in normal women or in several patients with other carcinomas, whereas 27 of 35 patients with cervical squamous cell carcinoma showed detectable serum antigen activity. All patients with advanced stages of cervical squamous cell carcinoma showed detectable antigen levels. These results indicate that there is a quantitative abnormality, at least, of this tumor antigen in patients with cervical squamous cell carcinoma and that the radioimmunoassay for the antigen is a potentially useful tool in clinical care.