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Relationship of a T‐Lymphocyte marker to phase of cell cycle and morphology of leukemic cells
Author(s) -
Arlin Zalmen A.,
Chiao Jen Wei,
Fried Jerrold,
Dowling Monroe D.,
Clarkson Bayard D.,
Good Robert A.
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197703)39:3<1101::aid-cncr2820390314>3.0.co;2-4
Subject(s) - bone marrow , mitosis , lymphoblast , pathology , vincristine , cell cycle , medicine , peripheral blood , lymphocyte , cell , biology , immunology , microbiology and biotechnology , cell culture , chemotherapy , cyclophosphamide , biochemistry , genetics
Spontaneous rosette formation with sheep erythrocytes (SRBC) was studied in the peripheral blood and bone marrow lymphoid cells from a patient whose leukemic cells appeared to be T‐lymphocytes. Simultaneous morphological examination of the peripheral blood white cells indicated that they consisted of 21% lymphoblasts; 26% prolymphocytes and 48% mature lymphocytes. The distribution of bone marrow cells within the cell cycle was determined by flow microfluorometry and 7 hours after treatment with vincristine consisted of 69% in G 1 , 21% in S, and 9% in mitosis. Since virtually all the cells both in marrow and blood formed rosettes with SRBC this implies that the expression of this T cell marker is independent both of the morphological appearance of these cells and their position within the cell cycle.