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Lacunar cells of nodular sclerosing Hodgkin's disease: An ultrastructural and immunohistologic study
Author(s) -
Anagnostou Dimitra,
Parker John W.,
Taylor Clive R.,
Chir B,
Tindle Barbara H.,
Lukes Robert J.
Publication year - 1977
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197703)39:3<1032::aid-cncr2820390306>3.0.co;2-x
Subject(s) - pathology , immunoperoxidase , lymphocyte , peripheral blood mononuclear cell , cytoplasm , reed–sternberg cell , medicine , antibody , biology , lymphoma , immunology , monoclonal antibody , microbiology and biotechnology , in vitro , biochemistry , hodgkin lymphoma
Tissues from 22 cases of nodular sclerosing Hodgkin's disease were studied by light and electron microscopy in conjunction with immunohistologic and cytochemical staining. The presence of lipid in the cytoplasm of lacunar cells suggested that this was responsible for the distinctive “lacunar” appearance of the cells. Marked morphologic similarities between “blast cells” resulting from mitogen stimulation of lymphocytes in vitro , immunoblasts seen in reactive lymphoid tissues, and mononuclear “Hodgkin's” cells in Hodgkin's disease suggested that all three cell types may result from lymphocyte transformation. It also seemed apparent that there was a developmental sequence from lymphocyte to transformed lymphocyte to the abnormal mononuclear Hodgkin's cell, with further progression, through increasing size and nuclear lobulation, to the lacunar cell or, alternatively, to the diagnostic Reed‐Sternberg cell. This proposed sequence was supported by immunoperoxidase studies in which cytoplasmic immunoglobulin was demonstrated in mononuclear Hodgkin's cells, lacunar cells and Reed‐Sternberg cells. The proposed relationship between these cells was also supported by the findings of both kappa and lambda chains in the same cells, a pattern not seen in reactive transformed lymphocytes.