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Morphologic and immunologic studies of a human colon tumor cell line (SW‐48)
Author(s) -
McCombs William B.,
Leibovitz Albert,
McCoy Cameron E.,
Stinson James C.,
Berlin Jerry D.
Publication year - 1976
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197612)38:6<2316::aid-cncr2820380619>3.0.co;2-0
Subject(s) - carcinoembryonic antigen , pathology , columnar cell , cell culture , adenocarcinoma , antigen , biology , medicine , microbiology and biotechnology , chemistry , epithelium , immunology , cancer , genetics
A permanent human tissue culture cell line (SW‐48) has been established from an adenocarcinoma of the transverse colon. Cells in the center of early colonies were cuboidal and loosely bound; cells on the periphery of the same colonies were more columnar, the nuclei were displaced toward the basal region, and the free surface formed stunted microvilli. These columnar cells often aligned themselves to resemble normal absorptive tissue. Carcinoembryonic antigen (CEA) was identified by immunofluorescent microscopy on the surface membrane of the tumor cells. Significantly more CEA could be isolated from the culture medium than from the whole cells. On agar gel diffusion analysis with a monospecific anti‐CEA serum (G.P. 32), SW‐48 CEA and CEA preparations from solid tumors demonstrated complete identity.