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An immunoelectron microscopic analysis of Epstein‐Barr virus‐associated complement‐fixing antigen
Author(s) -
Shamoto Mikihiro,
Suzuki Ikuo
Publication year - 1976
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197611)38:5<2057::aid-cncr2820380530>3.0.co;2-y
Subject(s) - antigen , immunoelectron microscopy , antibody , virus , complement (music) , virology , biology , complement fixation test , chemistry , microbiology and biotechnology , immunology , serology , biochemistry , gene , complementation , phenotype
Epstein‐Barr virus‐associated, complement‐fixing antigens have been observed with a complement‐mediating immunoreaction and with the peroxidase‐labeled anticomplement antibody electron microscopic method. Positive reaction products could be found in the nuclei of P3HR‐1 cell lines. At high magnification, it was ascertained that the reaction‐positive precipitates were associated with chromatin and were mostly either finely granular or filamentous, which suggests that the antigen was not uniform. It was speculated that the antigen would be analogous to the SV40 T‐antigen, since the localization pattern of the positive reaction was similar in both antigens. This new, modified method using complement may also be used for detection of a natural antibody of unknown class or for low‐sensitivity systems of antigen‐antibody reactions. Thus, this method appears useful for studying various kinds of experimental materials.