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Multiple molecular forms of lactate dehydrogenase and glucose 6‐phosphate dehydrogenase in normal and abnormal human breast tissues
Author(s) -
Hilf Russell,
Rector William D.,
Orlando Robert A.
Publication year - 1976
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197604)37:4<1825::aid-cncr2820370429>3.0.co;2-v
Subject(s) - fibroadenoma , lactate dehydrogenase , dehydrogenase , staining , fibrocystic breast disease , enzyme , glucose 6 phosphate dehydrogenase , fibrocystic disease , medicine , pathology , biochemistry , endocrinology , microbiology and biotechnology , chemistry , biology , breast cancer , cancer
Normal and abnormal breast samples of women were analyzed for multiple molecular forms of lactate dehydrogenase and glucose 6‐phosphate dehydrogenase, using acrylamide disc gel electrophoresis and specific enzyme staining techniques for separation and quantitation. Infiltrating ductal carcinomas demonstrated a significant increase (three‐to six‐fold) in the proportion of LDH‐5 compared to samples of normal breast, fibrocystic disease and fibroadenoma, indicative of a shift toward the muscle‐type lactate dehydrogenase in neoplasia. For glucose 6‐phosphate dehydrogenase, carcinomas were found to contain increased proportions of the fastest migrating species, G6PD‐I. Total enzyme activity/mg DNA was elevated in neoplastic tissues. Little or no alteration in isoenzyme profiles could be related to menopausal status of the patient.