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Characterization of an established cell line (SH‐3) derived from pleural effusion of patient with breast cancer
Author(s) -
Seman G.,
Hunter S. J.,
Miller R. C.,
Dmochowski L.
Publication year - 1976
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197604)37:4<1814::aid-cncr2820370428>3.0.co;2-1
Subject(s) - hela , cell culture , pleural effusion , karyotype , pathology , medicine , mycoplasma , alkaline phosphatase , cancer , effusion , cancer cell , cancer research , biology , enzyme , biochemistry , microbiology and biotechnology , genetics , surgery , gene , chromosome
Spontaneous emergence of fast‐growing cells in prolonged culture of pleural effusion cells obtained from a patient with breast cancer has led to the establishment of a cell line designated as SH‐3. By morphological criteria, as revealed by light and electron microscopy, SH‐3 cells are epithelial and resemble the poorly differentiated cells of a series of established human tumor cell lines. Their karyotype is hypotetraploid and different from that of HeLa cells. Isoenzyme analysis has shown the presence in the cells of a bone‐type alkaline phosphatase and of the fast (A) band of G6PD. The line is free of mycoplasma.