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Identification and subcellular localization of a sarcoma‐associated antigen(s) in a human cell line
Author(s) -
Lichtiger Benjamin,
Trujillo Jose M.,
Burk Kenneth H.,
Drewinko Benjamin
Publication year - 1976
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197604)37:4<1788::aid-cncr2820370425>3.0.co;2-v
Subject(s) - immunoperoxidase , sarcoma , antigen , antibody , immunofluorescence , subcellular localization , pathology , medicine , cell culture , cell , immunology , biology , microbiology and biotechnology , monoclonal antibody , cytoplasm , biochemistry , genetics
The present investigation was designed to demonstrate and identify the subcellular localization of sarcoma‐associated antigen(s) (TAA) in an established human neurogenic sarcoma line (T 2 cell line). Indirect immunofluorescence was used to screen sera from patients with sarcoma, nonsarcoma neoplasias, and from presumably normal blood bank donors. The positive sera were submitted to a series of absorptions to remove possible nonspecific cross‐reacting antibodies. The target cells were then studied with electron microscopy, utilizing the indirect immunoperoxidase technique to determine the subcellular localization of the sarcoma‐associated antigen(s). Findings indicate that sarcoma‐bearing patients have circulating antisarcoma antibodies directed to antigen(s) localized at the surface membrane of T 2 cells. These antibodies pertain to the IgM or IgG immunoglobulin class. The exact nature of the sarcoma‐associated antigen(s) identified in this study is not known at the present time. However, the results obtained strongly suggest that these substances may represent a new type of tumor‐associated antigen(s).