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Selective synthesis and accumulation of nuclear non‐histone proteins during carcinogenesis of the colon induced by 1, 2‐dimethylhydrazine
Author(s) -
Boffa Lidia C.,
Vidali Giorgio,
Allfrey Vincent G.
Publication year - 1975
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197512)36:6<2356::aid-cncr2820360614>3.0.co;2-m
Subject(s) - 1,2 dimethylhydrazine , histone , carcinogenesis , nuclear protein , carcinogen , dimethylhydrazine , biochemistry , biology , chemistry , microbiology and biotechnology , colorectal cancer , genetics , azoxymethane , cancer , dna , gene , transcription factor
The complement of nuclear non‐histone proteins in epithelial cells of the colon is progressively altered during the course of carcinogenesis induced by 1, 2‐dimethylhydrazine, until finally the nuclear proteins of tumor cells are easily distinguishable from those of the surrounding normal tissue. These changes in nuclear protein composition reflect earlier differences in the rates of synthesis of individual protein species. Radioisotopic double‐labeling experiments show that the synthesis of nuclear proteins of molecular weights 44,000 and 62,000 is selectively accelerated within 4 weeks after administration of the carcinogen, long before any morphological indications of malignancy appear.