Premium
Role of cyclic amp in differentiation of human neuroblastoma cells in culture
Author(s) -
Prasad Kedar N.,
Kumar S.
Publication year - 1975
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197510)36:4<1338::aid-cncr2820360422>3.0.co;2-1
Subject(s) - neurite , sodium butyrate , phosphodiesterase inhibitor , adenosine , phosphodiesterase , guanosine , endocrinology , medicine , cell culture , intracellular , programmed cell death , butyrate , neuroblastoma , biology , microbiology and biotechnology , biochemistry , apoptosis , enzyme , in vitro , genetics , fermentation
The inhibitors of cyclic AMP phosphodiesterase (papaverine and 4‐(‐3‐butoxy‐4‐methoxybenzyl)‐2‐imidazolidinone), serum‐free medium, and × irradiation caused cell death and neurite formation in human neuroblastoma cells in culture (IMR‐32), whereas theophylline was ineffective. Prostaglandin (PG)E 1 , N 6 O 2 ′‐dibutyryl adenosine 3′,5′‐cyclic monophosphate (dbcAMP) induced neurites without causing cell lethality. Inhibitors of phosphodiesterase and PGE 1 increased the intracellular level of cAMP by about 2‐ and 4‐fold respectively, whereas serum‐free medium and × irradiation did not. The combination of PGE 1 and phosphodiesterase inhibitor was more effective in causing morphological differentiation and in increasing the cAMP level than the individual agent. Sodium butyrate induced cell death and neurites, probably in part by increasing the cAMP level. cAMP, guanosine 3′,5′‐cyclic monophosphate, and adenosine had no detectable effect on the growth or morphology of neuroblastoma cells in culture. Adenosine 5′‐monophosphate produced cell death without causing neurite formation. DbcAMP, and to a much lesser degree, sodium butyrate increased the tyrosine hydroxylase activity.