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Cytotoxicity of human osteosarcoma sera to hamster sarcoma cells
Author(s) -
Pritchard Douglas J.,
Reilly Christopher A.,
Finkel Miriam P.,
Ivins John C.
Publication year - 1974
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197412)34:6<1935::aid-cncr2820340611>3.0.co;2-u
Subject(s) - osteosarcoma , hamster , cytotoxic t cell , sarcoma , cytotoxicity , medicine , antibody , immunology , pathology , cancer research , in vitro , biology , biochemistry
Previously, we reported the induction of osteosarcomas in Syrian hamsters by the injection of extracts of human osteosarcoma tissue. Evidence for interaction between human sera and a human‐induced hamster sarcoma was sought by use of a humoral microcytotoxicity test with the hamster sarcoma grown in tissue culture as the target cells, and sera obtained from 29 consecutive patients with osteosarcoma as the source of cytotoxic antibodies. Seventeen of the 29 human osteosarcoma sera showed marked cytotoxicity. Of the 16 patients who remained free of disease for at least 6 months, 15 had sera that were markedly cytotoxic, whereas of the 13 who developed metastases or died within 6 months of the test, only 2 had cytotoxic sera. Sera from 10 healthy persons were tested, and none was cytotoxic. This study offers further corroborative evidence for the transmission of a human osteosarcoma agent. In addition, it shows that patients without strongly positive humoral cytotoxic antibodies are likely to develop early metastasis or die.