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Soluble membrane antigens of human malignant melanoma cells
Author(s) -
Hollinshead Ariel C.,
Herberman Ronald B.,
Jaffurs William J.,
Alpert Louis K.,
Minton John P.,
Harris Jules E.
Publication year - 1974
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197410)34:4<1235::aid-cncr2820340433>3.0.co;2-k
Subject(s) - melanoma , sephadex , antigen , medicine , cell , pathology , immunology , chemistry , cancer research , biochemistry , enzyme
Delayed hypersensitivity reactions to soluble components of the cell membranes of autologous and allogeneic tumors were elicited in a series of patients with malignant melanoma. Two skin reactive antigens were prepared using stepwise low frequency sonication of cell membranes, chromatography of membrane sonicates on Sephadex G‐200, and separation by polyacrylamide gel electro‐phoresis (PAGE). One group of antigens, in Sephadex fraction II, PAGE region a, appears to be melanoma‐associated antigen. Sephadex fraction II, and further separated Sephadex fraction II PAGE region a, produced no reactions in 21 of 22 tests in patients with cancers other than melanoma; positive reactions were seen in 17 of 22 patients with early stage melanoma and in 7 of 19 patients with late stage melanoma. The other antigen, from Sephadex fraction III, PAGE region b, was also reactive in 5 of 6 early stage breast cancer patients. Comparable separated proteins from Sephadex fraction III, and Sephadex fraction III PAGE region b, of normal black skin cell membrane sonicates were reactive in 4 of 9 early stage melanoma patients but were negative in 7 late stage melanoma patients. Sephadex fraction III and Sephadex fraction III PAGE region b produced no reactions in patients with renal, cervical, colonic, and head and neck cancer; 9 of 21 patients with early stage melanomas were skin test positive; positive reactions were seen in 13 of 18 patients with late stage melanoma.