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Abnormal serum isoenzyme of leucine aminopeptidase (LAP) in malignant neoplastic disease
Author(s) -
Phillips Roswell W.,
Manildi Ernest R.
Publication year - 1974
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197408)34:2<350::aid-cncr2820340220>3.0.co;2-3
Subject(s) - medicine , aminopeptidase , malignancy , zymography , isozyme , leucine , gastroenterology , globulin , urine , abnormality , neoplasm , pathology , enzyme , endocrinology , biology , biochemistry , matrix metalloproteinase , amino acid , psychiatry
Abstract Leucine aminopeptidase (LAP) isoenzymes have been studied in the serum and urine of 53 consecutive patients with various types of malignant neoplastic disease, and compared with those in a group of 23 normal controls. Statistical evaluation and clinical correlation was made of the incidence and degree of zymogram abnormality and total serum and urine LAP levels in the patient group. LAP isoenzymes in normal sera separated into two principal fractions, one designated X, migrating in the alpha 2 globulin range, and Y, migrating in the beta globulin zone. Zymograms of the sera of patients with malignant neoplastic disease demonstrated a significant difference (p > 0.001) in the relative values of the X (alpha 2) and Y (beta) fractions, compared to the normal controls. A progressive increase in the Y fraction developed in those whose neoplastic malignancy worsened. A decrease in peak height of the Y fraction occurred during periods of remission. The study suggests that the LAP isoenzyme zymogram may be of value as a diagnostic aid and a method of assessing the degree of remission or progression of a neoplastic process.