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Demonstration of alpha‐fetoglobulin in hepatoma tissue by fluorescent antibody technique
Author(s) -
Chu Marie Lin,
Lin WenShihJen,
Yoshida Takato O.,
Chu ShuHsun,
Lin TienYu
Publication year - 1974
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197408)34:2<268::aid-cncr2820340208>3.0.co;2-x
Subject(s) - antiserum , immunodiffusion , globulin , ouchterlony double immunodiffusion , antibody , fluorescence , pathology , conjugate , radial immunodiffusion , medicine , gamma globulin , alpha (finance) , microbiology and biotechnology , immunofluorescence , alpha globulin , chemistry , immunology , biology , biochemistry , mathematical analysis , construct validity , nursing , gene , patient satisfaction , physics , mathematics , quantum mechanics
The fluorescent antibody technique with specific conjugate of antiserum to alpha‐fetoglobulin was applied to five hepatoma tissues, of which three cases showed positive α‐f globulin identified by a modified Ouchterlony immunodiffusion technique; two cases were negative. Various histologic patterns of tumor tissue were present both in α‐f globulin positive and negative cases. The intensity of the total area of fluorescence in tumor tissue was found to be well correlated with the quantitative aspects of serum α‐f globulin, but bore no relationship to the various histologic patterns of tumor. The two negative serum α‐f globulin cases also showed small limited areas of faint fluorescence in tumor tissue.