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Pathogenesis of a transplanted canine lymphocytic leukemia
Author(s) -
Cohen Hilliard,
Chapman Albert L.,
Bopp William J.,
Schmidt Charlotte E.,
Przybylski Connie E.,
McPhee Mark S.
Publication year - 1974
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197405)33:5<1313::aid-cncr2820330516>3.0.co;2-4
Subject(s) - spleen , beagle , lymph , medicine , bone marrow , pathology , leukemia , buffy coat , lymphatic system , immunology
Eight neonatal beagles were inoculated with buffy coat cells of a canine lymphocytic leukemia maintained in serial passage in beagle neonates. Animals were killed on days 1, 2, 4, 7, 11, 16, 20, and 22 after inoculation. The first appearance of lymphocytic neoplasm was noted on day 4 at the inoculation site and in two regional lymph nodes. By day 11, leukemic cells had infiltrated other lymph nodes, bone marrow, and spleen. On day 16 the liver was infiltrated, and the peripheral blood was leukemic. The results of this study suggest that the leukemic cells initially spread via the lymphatics to lymph nodes and subsequently by the blood stream to bone marrow and spleen; the liver was involved secondarily to the spleen via the portal venous system. The peripheral blood did not contain large numbers of circulating neoplastic lymphocytes until after bone marrow and spleen had already demonstrated considerable leukemic invasion. The finding of karyotypically female cells in a male recipient suggests that the leukemia was derived from the female donor and was therefore a transplant.