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Pathogenesis of hodgkin's disease: Separation and culture of different kinds of cells from hodgkin's disease in a sterile isokinetic gradient of ficoll in tissue culture medium
Author(s) -
Pretlow Thomas G.,
Luberoff Douglas E.,
Hamilton Linda J.,
Weinberger Phillip C.,
Maddox William A.,
Durant John R.
Publication year - 1973
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197305)31:5<1120::aid-cncr2820310513>3.0.co;2-1
Subject(s) - ficoll , tissue culture , medicine , pathogenesis , disease , pathology , immunology , biology , in vitro , genetics , peripheral blood mononuclear cell
The pathogenesis of Hodgkin's disease is controversial. Some view the lymphocyte in Hodgkin's tumor as an integral part of the malignant proliferation; others interpret the lymphocyte as a manifestation of the host's attempt to reject the neoplasm. In an effort to study interactions between individual kinds of cells from Hodgkin's disease, we have developed a method for disaggregating Hodgkin's tumor using collagenase. The disaggregated cells have been frozen in a viable state and, after storage over liquid nitrogen, two kinds of cells have been obtained consistently in tissue culture from all five patients whose cells have been studied. Prior to culture, one of these cell types—a very homogeneous population of mature‐appearing lymphoid cells—has been separated from the other kinds of cells in 98.4 ± O.4% purity. This separation was accomplished using centrifugation in a gradient of Ficoll (polysucrose) in tissue culture medium. In culture, these cells divide and produce only lymphoid cells. The other kind of cell which can be cultured from Hodgkin's disease—a histiocytic cell—can be widely separated from the lymphoid cells and does not give rise to lymphocytes in tissue culture. Using purified populations of the individual kinds of cells, it will be possible to study the possible cytotoxicity of the lymphoid cells for the histiocytic cells. To our knowledge, this is the first reported separation of lymphocytes from solid tumors, and the method may be broadly applicable in the investigation of lymphocytes which infiltrate solid tumors.