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Medullary carcinoma of thyroid gland. Subcellular distribution of calcitonin and relationship between granules and amyloid
Author(s) -
Meyer John S.,
Hutton William E.,
Kenny Alexander D.
Publication year - 1973
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197302)31:2<433::aid-cncr2820310221>3.0.co;2-f
Subject(s) - calcitonin , medullary carcinoma , thyroid , amyloid (mycology) , medullary cavity , microsome , pathology , granule (geology) , ultracentrifuge , endocrinology , medicine , chemistry , biochemistry , thyroid carcinoma , biology , in vitro , paleontology
Electron microscopy and bioassay for calcitonin were used to study two intact medullary carcinomas and their subcellular fractions. The fractions were prepared by sucrose gradient ultracentrifugation after removal of mitochondria. Virtually all of the calcitonin was bound to subcellular particles, but it was distributed among all of the particulate fractions that were obtained, and consequently it cannot be assigned to any one kind of particle. The two types of particles with which calcitonin most probably is associated are secretory granules and microsomes. Secretory granules also were closely related to the amyloid of the medullary carcinoma, and we suggest that the granules play a role in formation of the amyloid.