Premium
Production of interferon induced by hyaluronic acid from Rous sarcoma
Author(s) -
Watanabe Masao,
Sato Mitsunobu
Publication year - 1972
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197202)29:2<517::aid-cncr2820290243>3.0.co;2-n
Subject(s) - rous sarcoma virus , fractionation , interferon , inducer , biochemistry , microbiology and biotechnology , hyaluronidase , biology , chemistry , enzyme , virology , chromatography , gene
It has been suggested that HA in the Rous sarcoma has an inducing activity to produce IF‐like inhibitor in the chick embryo cell cultures; this has been studied further by us. The IF‐like inhibitor produced was shown to have cell species specificity, but not to have virus specificity, and both the production and the activity of the inhibitor were shown to be inhibited by actinomycin D. These properties of the inhibitor, as well as the acid resistance, protease sensitivity, etc., showed that the inhibitor was interferon itself. The molecular weight of the IF produced was estimated to be 105,000, 68,000, and 28,000. The inducer in the phenol extract of Rous sarcoma was purified by CTAB fractionation, and nucleic acid‐free HA fraction was obtained. This fraction was shown to have IF‐inducing activity, which was inactivated by hyaluronidase treatment. These properties of the inducer, as well as the behavior in the fractionation with Kirby's and Westphal's methods, and chemical analysis data, show that the inducer is HA of special nature different from that of human umbilical cord or normal chorioallantoic membrane of chicken egg. The molecular weight of the Rous HA was shown to have the main peak at 5 × 10 5 by gel filtration. Rous HA was shown to have a straight dose reponse for the IF‐inducing capacity until 10 μg/ml and plateau over this value, and DEAE dextran had an enhancing effect on the interferon induction of Rous HA.