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Evaluation of the mode of cell death in ehrlich ascites tumor
Author(s) -
Lala Peeyush K.
Publication year - 1972
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197201)29:1<261::aid-cncr2820290139>3.0.co;2-z
Subject(s) - mitosis , ascites , cell cycle , cell , thymidine , cell division , programmed cell death , interphase , pathology , medicine , biology , microbiology and biotechnology , biochemistry , apoptosis , in vitro
Since there was a progressive transition of proliferating cells into a non‐proliferating resting state, as well as an increased rate of cell loss in the aging ascites tumor, we examined whether cell loss preferentially affected the non‐proliferating or the proliferating cells. The kinetics of cell loss in the 7‐day ascites tumor obtained with inoculation of 10 6 cells was evaluated by repeated labeling with H 3 ‐thymidine (H 3 TdR), combined with the measurements of cell cycle characteristics by the labeled mitosis technique after a single injection of H 3 TdR. The median cell cycle time was 24 hours (S period = 20 hours, G 2 + mitosis = 4 hours, and there was no measurable G 1 ), and the rate of cell production approached the rate of cell loss at this stage. The absence of G 1 was verified with a double‐labeling technique. On the basis of these data, the temporal changes in the labeling indices of tumor cells, tumor cells in mitosis, and figures resembling degenerating mitoses following repeated H 3 TdR injection could be best explained by an age‐specific elimination of non‐cycling cells and a small amount of mitotic death.