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Transformation of human cells by virus and chemical carcinogen in vitro
Author(s) -
Sekiya Souei
Publication year - 1971
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197109)28:3<789::aid-cncr2820280339>3.0.co;2-i
Subject(s) - rous sarcoma virus , cheek pouch , in vitro , virus , hamster , embryo , virology , malignant transformation , tissue culture , carcinogen , cell culture , biology , viral transformation , microbiology and biotechnology , cancer research , biochemistry , genetics
In vitro transformation of human embryo and uterine myoma cells was attempted by the Schmidt‐Ruppin strain of Rous sarcoma virus (SR‐RSV). In addition to single SR‐RSV treatment, the combined effect of SR‐RSV and 3‐methylcholanthrene (MC) to human embryo cells was examined. Morphological transformation was observed within 3–4 weeks after SR‐RSV infection. On the other hand, infectious virus was not detected in culture fluid, but viral genome was detected in all the SR‐RSV infected cultures despite single or double treatment. The non‐treated cultures and cultures treated with SR‐RSV alone failed to produce a tumor in cortisone‐conditioned hamster cheek pouch under the conditions of our experiments, but cultures treated with both MC and SR‐RSV produced histologically malignant tumors. However, the growth rate of transformed human cells was not different from that of non‐treated controls, and a stable line of transformed human cells could not be established in vitro.