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Tumor hemorrhagic properties of surface components of salmonella typhimurium mutants
Author(s) -
Wasilauskas Benedict L.,
Cameron J. A.
Publication year - 1971
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197101)27:1<217::aid-cncr2820270130>3.0.co;2-p
Subject(s) - lipid a , mutant , microbiology and biotechnology , antigen , enterobacteriaceae , salmonella , necrosis , biology , bacteria , medicine , biochemistry , escherichia coli , lipopolysaccharide , immunology , pathology , gene , genetics
A number of bacterial components were tested for their ability to cause hemorrhagic necrosis of Sarcoma 37 in mice. These components included the common antigen found in the Enterobacteriaceae, a high molecular weight glucan composed of alpha and beta glucose, and LPS preparation of varying structural complexity. Common antigen and glucan were found to be inactive. All LPS preparations prepared by phenol water extraction were found to cause necrosis to approximately the same extent. The simplest LPS (chemotype Re) contains only 2 keto‐3‐deoxyoctonate and a lipid moiety. Isolation of these 2 components resulted in a loss of activity, suggesting the necessity of a specific molecular configuration to elicit the tumor hemorrhagic response. LPS extracted from Re mutants by EDTA showed no tumor hemorrhagic activity, while similar extracts of wild type organisms did show activity. These results suggest that the LPS of Re organisms is not a surface component.