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Investigation of organ culture as an aid to the hormonal management of breast cancer
Author(s) -
Stoll Basil A.
Publication year - 1970
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(197005)25:5<1228::aid-cncr2820250531>3.0.co;2-c
Subject(s) - organ culture , breast cancer , acid phosphatase , medicine , hormone , progestin , alkaline phosphatase , cancer , estrogen , enzyme , physiology , incubation , andrology , endocrinology , biochemistry , biology , in vitro
Specimens of breast cancer from 31 patients were incubated for 24 to 48 hours in organ culture. The effect of exposure to various concentrations of estrogen or progestin could be demonstrated histochemically by the lysosomal fragility test, using acid phosphatase as the marker enzyme. Neither the total lactic dehy‐drogenase nor the ratio between the M and H subunits appeared to be significantly affected by incubation of the organ culture with steroids at the concentrations reported. The problems of using organ cultures of breast cancer and histochemical assay of enzyme distribution as an aid to the hormonal management of the disease are discussed.