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Defective photochemical repair in epithelium predisposed to field cancerization
Author(s) -
Roth Daniel,
Sage Harold H.
Publication year - 1969
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(196909)24:3<511::aid-cncr2820240312>3.0.co;2-n
Subject(s) - pyrimidine dimer , dna repair , dna , dna damage , wi 38 , buccal administration , ultraviolet light , medicine , biology , chemistry , photochemistry , biochemistry , ploidy , pharmacology , gene
Capability for repairing ultraviolet radiation damage was studied in the desquamated buccal cells of 34 individuals with histories of cancer of the oral cavity and upper respiratory tract, and of 3 asymptomatic smokers. Repair was evaluated in terms of the cellular uptake of an acridine marker for thymine dimer, in response to visible light and an enzyme derived from baker's yeast possessing photoreactivating ability (cytochrome b2). A repair deficit was displayed by the tested cells regardless of disease stage, treatment, or cure. Cultured diploid human cells which had been incubated with calf thymus DNA or Simian virus 40 developed a comparable repair deficit showing both dose‐relationship and specificity for the incubated DNA. It is proposed that incorporated DNA, by failing to respond adequately to repair mechanisms, may increase the cell's sensitivity to mutagenic agents.